rabbit polyclonal anti cdk6 antibody  (Proteintech)

Journal: iScience

Article Title: Cdk6’s functions are critically regulated by its unique C-terminus

doi: 10.1016/j.isci.2024.111697

Figure Lengend Snippet:

Article Snippet: Rabbit polyclonal anti-CDK6 , Santa Cruz Biotechnology , Cat# sc-7180; RRID: AB_2076998.

Techniques: Recombinant, Luciferase, Magnetic Beads, Immunoprecipitation, Bicinchoninic Acid Protein Assay, Mass Spectrometry, Modification, cDNA Synthesis, SYBR Green Assay, Western Blot, Bradford Protein Assay, Software

Journal: Molecular cell

Article Title: Phosphorylated RB Promotes Cancer Immunity by Inhibiting NF-κB Activation and PD-L1 Expression

doi: 10.1016/j.molcel.2018.10.034

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: Rabbit polyclonal anti-CDK6 , Santa Cruz , Cat# sc-177; RRID: AB_631225.

Techniques: Virus, Recombinant, Control, Mutagenesis, Kinase Assay, Sequencing, CRAfT Assay, Software

Journal: Cell host & microbe

Article Title: CRISPR/Cas9 Screens Reveal Epstein-Barr Virus Transformed B-Cell Host Dependency Factors

doi: 10.1016/j.chom.2017.04.005

Figure Lengend Snippet: (A) Scatter plot of EBV-mediated effects on primary human B cell gene expression Y axis, Log2 (LCL to primary B-cell ratios); X axis, −log10 (p-value). Values (Price et al., 2012) of top LCL-selective hits (red circle) versus all other B cell genes (blue circle) are shown. (B) Ratios of EBV-induced to EBV-suppressed primary B-cell host genes for genome-wide (gray bar) versus for the 87 LCL-selective screen hits at q <0.05 cutoff (black bar). (C) CRISPR screens identified distinct GM12878 and P3HR1 cyclin/cyclin-dependent kinase dependencies. Data are expressed as Log2 normalized values of sgRNA abundance from quadruplicate screens. The four sgRNAs against CDK4 (triangle), CCND3 (square), CDK6 (closed circle) and CCND2 (diamond) are highlighted among all Avana sgRNA library abundances (open circle). (D) Immunoblot analysis of CDK4 or CDK6 knockout in whole cell extracts of GM12878 or P3HR1 with the indicated sgRNAs. (E-F) Growth curves of GM12878 versus P3HR1 Cas9 cells expressing CDK6 (red), CDK4 (blue) or non-targeting control (black) sgRNA. Mean and standard deviation (S.D.) of triplicate experiments are shown. See also Figure S1.

Article Snippet: Rabbit polyclonal anti-CDK6 antibody , Bethyl , Cat#A304-937A; RRID: AB_2621131.

Techniques: Gene Expression, Genome Wide, CRISPR, Western Blot, Knock-Out, Expressing, Control, Standard Deviation

Journal: Cell host & microbe

Article Title: CRISPR/Cas9 Screens Reveal Epstein-Barr Virus Transformed B-Cell Host Dependency Factors

doi: 10.1016/j.chom.2017.04.005

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: Rabbit polyclonal anti-CDK6 antibody , Bethyl , Cat#A304-937A; RRID: AB_2621131.

Techniques: Purification, Virus, Recombinant, SYBR Green Assay, Cell Viability Assay, Caspase-Glo Assay, Isolation, Cell Culture, Control, CRISPR, Software

Journal: Frontiers in Cell and Developmental Biology

Article Title: Roles of Melatonin in Goat Hair Follicle Stem Cell Proliferation and Pluripotency Through Regulating the Wnt Signaling Pathway

doi: 10.3389/fcell.2021.686805

Figure Lengend Snippet: Information of primers for real-time quantitative PCR.

Article Snippet: Cyclin-dependent kinase 6 , Anti-CDK6 rabbit polyclonal antibody , Sangon Biotech , D120398.

Techniques: Binding Assay

Journal: Frontiers in Cell and Developmental Biology

Article Title: Roles of Melatonin in Goat Hair Follicle Stem Cell Proliferation and Pluripotency Through Regulating the Wnt Signaling Pathway

doi: 10.3389/fcell.2021.686805

Figure Lengend Snippet: List of antibodies used for Immunofluorescence analysis and western blot.

Article Snippet: Cyclin-dependent kinase 6 , Anti-CDK6 rabbit polyclonal antibody , Sangon Biotech , D120398.

Techniques: Immunofluorescence, Western Blot, Binding Assay, Recombinant

Journal: Frontiers in Cell and Developmental Biology

Article Title: Roles of Melatonin in Goat Hair Follicle Stem Cell Proliferation and Pluripotency Through Regulating the Wnt Signaling Pathway

doi: 10.3389/fcell.2021.686805

Figure Lengend Snippet: Melatonin promoted the proliferation and cell cycle of gsHFSCs by promoting Wnt downstream factor expression, ∗∗∗ p < 0.001. (A) MTT analysis of melatonin on gsHFSCs proliferation following 72 h of exposure. (B) Cell cycle analysis of gsHFSCs after melatonin exposure at 72 h. (C) Relative mRNA level of C-JUN , C-MYC, CYCLIND1 , and CDK6 as detected by RT-qPCR after melatonin exposure for 72 h; ∗∗ p < 0.01, ∗ p < 0.05 (Control: green; 72 h melatonin: blue). (D) Proteins tested by immunofluorescence staining. Scale bar, 50 μm.

Article Snippet: Cyclin-dependent kinase 6 , Anti-CDK6 rabbit polyclonal antibody , Sangon Biotech , D120398.

Techniques: Expressing, Cell Cycle Assay, Quantitative RT-PCR, Control, Immunofluorescence, Staining